1,314 research outputs found
Phosphoproteomic Analyses of Interleukin 2 Signaling Reveal Integrated JAK Kinase-Dependent and -Independent Networks in CD8+ T Cells
SummaryInterleukin-2 (IL-2) is a fundamental cytokine that controls proliferation and differentiation of T cells. Here, we used high-resolution mass spectrometry to generate a comprehensive and detailed map of IL-2 protein phosphorylations in cytotoxic T cells (CTL). The data revealed that Janus kinases (JAKs) couple IL-2 receptors to the coordinated phosphorylation of transcription factors, regulators of chromatin, mRNA translation, GTPases, vesicle trafficking, and the actin and microtubule cytoskeleton. We identified an IL-2-JAK-independent SRC family Tyr-kinase-controlled signaling network that regulates ∼10% of the CTL phosphoproteome, the production of phosphatidylinositol (3,4,5)-trisphosphate (PIP3), and the activity of the serine/threonine kinase AKT. These data reveal a signaling framework wherein IL-2-JAK-controlled pathways coordinate with IL-2-independent networks of kinase activity and provide a resource toward the further understanding of the networks of protein phosphorylation that program CTL fate
Towards understanding balancing in exertion games
Playing exertion games with others can be engaging. However, players with different physical skill levels competing against each other can experience reduced engagement because they are either not challenged enough, or challenged too much. Balancing methods can address this; however, there is only limited understanding of balancing in exertion games. In this paper, we identify two distinct dimensional balancing techniques: "internal adjustment" and "external adjustment". We report results from a study where we measured player engagement after applying these adjustments to a digital table tennis game and the traditional table tennis game, finding two disengagement factors: "unexpected physical challenges" and "unacceptable competitive advantage". Based on these factors we derived a set of exertion game design considerations. We conclude that applying digital technology to a physical game can change the required skill level to play the game, and this can affect the impact of these adjustments on player engagement. These results enhances our understanding of balancing in exertion games, supporting the benefits of playing exertion games with others
Synthesis and characterization of atomically-thin graphite films on a silicon carbide substrate
This paper reports the synthesis and detailed characterization of graphite
thin films produced by thermal decomposition of the (0001) face of a 6H-SiC
wafer, demonstrating the successful growth of single crystalline films down to
approximately one graphene layer. The growth and characterization were carried
out in ultrahigh vacuum (UHV) conditions. The growth process and sample quality
were monitored by low-energy electron diffraction, and the thickness of the
sample was determined by core level x-ray photoelectron spectroscopy.
High-resolution angle-resolved photoemission spectroscopy shows constant energy
map patterns, which are very sharp and fully momentum-resolved, but nonetheless
not resolution limited. We discuss the implications of this observation in
connection with scanning electron microscopy data, as well as with previous
studies
Thickness Estimation of Epitaxial Graphene on SiC using Attenuation of Substrate Raman Intensity
A simple, non-invasive method using Raman spectroscopy for the estimation of
the thickness of graphene layers grown epitaxially on silicon carbide (SiC) is
presented, enabling simultaneous determination of thickness, grain size and
disorder using the spectra. The attenuation of the substrate Raman signal due
to the graphene overlayer is found to be dependent on the graphene film
thickness deduced from X-ray photoelectron spectroscopy and transmission
electron microscopy of the surfaces. We explain this dependence using an
absorbing overlayer model. This method can be used for mapping graphene
thickness over a region and is capable of estimating thickness of multilayer
graphene films beyond that possible by XPS and Auger electron spectroscopy
(AES).Comment: 14 pages, 9 figure
Substrate-induced band gap opening in epitaxial graphene
Graphene has shown great application potentials as the host material for next
generation electronic devices. However, despite its intriguing properties, one
of the biggest hurdles for graphene to be useful as an electronic material is
its lacking of an energy gap in the electronic spectra. This, for example,
prevents the use of graphene in making transistors. Although several proposals
have been made to open a gap in graphene's electronic spectra, they all require
complex engineering of the graphene layer. Here we show that when graphene is
epitaxially grown on the SiC substrate, a gap of ~ 0.26 is produced. This gap
decreases as the sample thickness increases and eventually approaches zero when
the number of layers exceeds four. We propose that the origin of this gap is
the breaking of sublattice symmetry owing to the graphene-substrate
interaction. We believe our results highlight a promising direction for band
gap engineering of graphene.Comment: 10 pages, 4 figures; updated reference
Guidelines for training in cardiovascular magnetic resonance (CMR)
These "Guidelines for training in Cardiovascular Magnetic Resonance" were developed by the Certification Committee of the Society for Cardiovascular Magnetic Resonance (SCMR) and approved by the SCMR Board of Trustees
Surface Chemistry Involved in Epitaxy of Graphene on 3C-SiC(111)/Si(111)
Surface chemistry involved in the epitaxy of graphene by sublimating Si atoms from the surface of epitaxial 3C-SiC(111) thin films on Si(111) has been studied. The change in the surface composition during graphene epitaxy is monitored by in situ temperature-programmed desorption spectroscopy using deuterium as a probe (D2-TPD) and complementarily by ex situ Raman and C1s core-level spectroscopies. The surface of the 3C-SiC(111)/Si(111) is Si-terminated before the graphitization, and it becomes C-terminated via the formation of C-rich (6√3 × 6√3)R30° reconstruction as the graphitization proceeds, in a similar manner as the epitaxy of graphene on Si-terminated 6H-SiC(0001) proceeds
Female firesetters: gender associated psychological and psychopathological features
Objective: Female firesetters are reported to commit nearly a third of deliberately set fires, yet there are limited studies examining the characteristics that distinguish them from suitable comparison groups. The aim of this study is to compare incarcerated female firesetters with incarcerated male firesetters and female offender controls on psychopathological and psychological features that could be targeted via therapeutic interventions.
Method: Sixty-five female firesetters, 128 male firesetters, and 63 female offenders were recruited from the prison estate. Participants completed a battery of validated tools assessing psychiatric traits and psychological characteristics (i.e., inappropriate fire interest,
emotion/self-regulation, social competence, self-concept, offense-supportive attitudes, and boredom proneness) highlighted in the existing literature.
Results: Major depression and an internal locus of control distinguished female firesetters from male firesetters. Alcohol dependence, serious/problematic fire interest, and more effective anger regulation distinguished female firesetters from the female offender control group.
Conclusions: This is the first study to examine differences between female firesetters, male firesetters, and female control offenders on both psychopathological features and psychological traits. These findings highlight the gender-specific and offence-specific needs of female firesetters that clinicians need to consider when implementing programs that ensure client responsivity.
Keywords: firesetting, arson, psychopathology, female offender
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